Description
TurboTag 415 Protein Labeling Kit for High-Performance DY-415 Conjugation
TurboTag 415 from emp BIOTECH is a complete labeling and purification kit for covalent conjugation of proteins with DY-415 fluorophore. The kit is ideal for labeling proteins ≥ 25 kDa—especially antibodies—using a highly reactive NHS ester derivative of DY-415 dye. The resulting conjugates exhibit strong fluorescence with excitation/emission maxima at 418 nm and 467 nm, enabling sensitive detection in biochemical and immunoassay workflows.
With each vial containing 50 nmol of reactive dye, up to 15 nmol of protein can be labeled. TurboTag 415 provides a robust, reproducible labeling process through stable amide linkage formation, and is compatible with standard IgG labeling protocols for 1 mg and 100 µg samples.
Performance Highlights
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Fast, efficient labeling of proteins ≥ 25 kDa, especially IgG and other antibodies
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Strong fluorescent signal with DY-415 (excitation/emission at 418/467 nm)
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Covalent labeling via NHS ester chemistry for high stability
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Compatible with pre-optimized protocols for 1 mg or 100 µg IgG
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Kit includes dye and materials for purification in a convenient format
Properties
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Molecular Weight: 573.56
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Empirical Formula: C₂₄H₂₈N₃O₁₀SNa
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Absorption [λmax]: 418 ± 3 nm
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Emission [λmax]: 467 ± 3 nm
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Extinction Coefficient (ε): 34,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and proteins
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Protein tracking in immunoassays and detection platforms
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Biomarker detection and protein-based diagnostics
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High-sensitivity imaging and signal amplification studies
Why Choose TurboTag™ 415?
TurboTag 415 delivers fast, reproducible fluorescent labeling with DY-415 for demanding analytical applications. Designed for proteins ≥ 25 kDa, its NHS ester chemistry ensures stable, covalent conjugation under mild conditions. With strong absorption and emission in the blue spectral range, this kit supports sensitive detection in fluorescence-based workflows. The pre-optimized format minimizes hands-on time, allowing researchers to achieve consistent, high-quality protein-dye conjugates in under an hour.
