Description
TurboTag 405 Protein Labeling Kit for High-Performance DY-405 Conjugation
TurboTag 405 from emp BIOTECH is a complete labeling and purification kit for covalent conjugation of proteins with DY-405 fluorophore. Designed for proteins ≥ 25 kDa—particularly antibodies—the kit features a highly reactive NHS ester derivative of DYOMICS’ DY-405 dye. The resulting conjugates exhibit excitation/emission maxima at 400 nm and 423 nm, offering sharp, high-contrast fluorescence for sensitive detection in biochemical and immunoassay workflows.
Each kit contains 50 nmol of reactive dye, sufficient to label up to 15 nmol of protein. TurboTag 405 supports rapid, efficient labeling through amide bond formation and is compatible with emp BIOTECH’s pre-optimized protocols for labeling 1 mg or 100 µg of IgG.
Performance Highlights
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Reliable fluorescent labeling of proteins ≥ 25 kDa, especially antibodies
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Strong DY-405 signal with excitation/emission at 400/423 nm
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NHS ester chemistry for stable amide linkage with primary amines
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Label up to 15 nmol of protein with each 50 nmol dye vial
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Compatible with standard IgG labeling protocols (1 mg and 100 µg formats)
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Rapid workflow with integrated labeling and purification steps
Properties
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Molecular Weight: 792.66
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Empirical Formula: C₂₈H₂₃N₂O₁₅S₃Na₃
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Absorption [λmax]: 400 ± 3 nm
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Emission [λmax]: 423 ± 3 nm
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Extinction Coefficient (ε): 32,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: –20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
- Fluorescent labeling of proteins and antibodies
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Signal detection in immunoassays and fluorescence-based assays
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Analytical labeling of proteins for tracking, imaging, or quantification
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Use in high-throughput biomolecular diagnostics and R&D platforms
Why Choose TurboTag™ 405?
TurboTag 405 combines the bright fluorescence of DY-405 with robust NHS ester reactivity to deliver reliable protein labeling under mild conditions. Optimized for proteins ≥ 25 kDa, the kit enables rapid and reproducible conjugation workflows for sensitive detection and imaging. Its efficient labeling capacity and blue-violet emission profile make it a powerful tool for scientists conducting fluorescence-based analysis, immunodetection, and protein tracking.
