Description
TurboTag 647 Protein Labeling Kit for Robust DY-647 Conjugation
TurboTag 647 from emp BIOTECH is a comprehensive protein labeling and purification kit tailored for covalent conjugation of antibodies and other proteins ≥ 25 kDa using DY-647, a far-red fluorophore. The included reactive NHS ester dye forms a stable amide bond with primary amines on proteins, delivering reliable, reproducible conjugation for fluorescence-based workflows.
Each vial provides 50 nmol of reactive dye and allows labeling of up to 15 nmol of protein. The resulting conjugates exhibit sharp excitation/emission peaks at 653 nm and 672 nm, ideal for sensitive detection in multiplex assays, in vivo studies, and fluorescence microscopy. TurboTag 647 comes with optimized protocols for both 1 mg and 100 µg IgG labeling.
Performance Highlights
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Efficient labeling of proteins ≥ 25 kDa, especially IgG and antibodies
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Far-red fluorescence with excitation at 653 nm and emission at 672 nm
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Stable amide linkage formation via NHS ester chemistry
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Pre-validated for 1 mg and 100 µg IgG samples
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High brightness and photostability for sensitive detection
Properties
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Molecular Weight: 805.91
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Empirical Formula: C₃₈H₄₄N₃O₁₁S₂Na
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Absorption [λmax]: 653 ± 3 nm
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Emission [λmax]: 672 ± 3 nm
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Extinction Coefficient (ε): 250,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with 1° amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies
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Far-red detection in multiplexed fluorescence assays
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Flow cytometry, microscopy, and imaging
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Immunohistochemistry and Western blotting
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High-sensitivity biochemical analysis and diagnostics
Why Choose TurboTag™ 647?
TurboTag 647 offers robust protein labeling performance with DY-647, a bright far-red fluorophore suited for advanced fluorescence applications. Its NHS ester chemistry ensures consistent conjugation with high signal intensity and minimal background. Optimized protocols for small and large sample sizes support flexibility across research and diagnostic settings. Whether for imaging, assay development, or antibody tracking, TurboTag 647 delivers reliable results in minutes.
