Description
TurboTag 495 Protein Labeling Kit for High-Performance DY-495 Conjugation
TurboTag 495 from emp BIOTECH is a complete labeling and purification kit for covalent conjugation of proteins with DY-495 fluorophore. Designed for labeling proteins ≥ 25 kDa—especially antibodies—the kit uses a highly reactive NHS ester of DY-495 to form stable amide linkages under mild conditions. The resulting conjugates exhibit fluorescence excitation at 493 nm and emission at 521 nm, providing strong and reliable signal for fluorescence-based applications.
Each vial contains 50 nmol of reactive dye, sufficient for labeling up to 15 nmol of protein. TurboTag 495 is compatible with emp BIOTECH’s optimized labeling protocols for 1 mg and 100 µg of IgG, enabling a reproducible workflow with high labeling efficiency.
Performance Highlights
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Rapid and efficient labeling of proteins ≥ 25 kDa, ideal for antibodies and enzymes
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Bright fluorescence with DY-495 (excitation/emission at 493/521 nm)
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Stable, covalent labeling via NHS ester chemistry
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Labels up to 15 nmol protein per 50 nmol dye vial
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Pre-validated protocols available for 1 mg or 100 µg of IgG
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Excellent choice for sensitive detection in diagnostic and imaging workflows
Properties
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Molecular Weight: 623.02
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Empirical Formula: C₃₁H₂₇N₂O₁₀Cl
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Absorption [λmax]: 493 ± 3 nm
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Emission [λmax]: 521 ± 3 nm
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Extinction Coefficient (ε): 70,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and other proteins
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Immunoassays, blotting, and imaging requiring strong green fluorescence
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Protein tracking in cell-free systems and in vitro diagnostics
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High-sensitivity detection in analytical assays
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Bioconjugation workflows using NHS chemistry
Why Choose TurboTag™ 495?
TurboTag 495 enables fast, high-yield fluorescent labeling of proteins with DY-495, a dye offering bright emission in the green spectrum. Its optimized NHS ester chemistry ensures reproducible conjugation with minimal hands-on time. Whether for diagnostic assays, fluorescence imaging, or protein detection, TurboTag 495 delivers robust, high-signal labeling performance for proteins ≥ 25 kDa—empowering researchers with a reliable solution for precise and sensitive bioconjugation.
