Description
TurboTag 682 Protein Labeling Kit for High-Sensitivity Near-Infrared Detection
TurboTag 682 from emp BIOTECH is a robust protein labeling and purification kit designed for efficient covalent conjugation of proteins using the near-infrared fluorophore DY-682. Ideal for labeling proteins ≥ 25 kDa—especially monoclonal antibodies—the kit uses a highly reactive NHS ester of DY-682 to create stable amide linkages with primary amines. The resulting conjugates deliver strong fluorescence with excitation/emission maxima at 692 nm and 709 nm, enabling highly sensitive detection with minimal background in imaging and analytical workflows.
Each kit provides 50 nmol of reactive dye, allowing labeling of up to 15 nmol of protein. Ready-to-use protocols for 1 mg or 100 µg of IgG make TurboTag 682 a reliable tool for quick and reproducible fluorescent labeling in demanding research and diagnostic applications.
Performance Highlights
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Efficient labeling of proteins ≥ 25 kDa, including monoclonal antibodies
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Near-infrared fluorescence (excitation/emission: 692/709 nm) for low-background detection
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Stable covalent bond formation via NHS ester chemistry
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High extinction coefficient (140,000 M⁻¹·cm⁻¹) for strong fluorescence signal
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Includes optimized protocols for small and medium IgG labeling
Properties
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Molecular Weight: 950.03
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Empirical Formula: C₄₁H₄₉N₃O₁₄S₃Na₂
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Absorption [λmax]: 692 ± 3 nm
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Emission [λmax]: 709 ± 3 nm
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Extinction Coefficient (ε): 140,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol of protein per 50 nmol dye vial
Applications
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Antibody and protein labeling for near-infrared fluorescence
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Multiplex immunoassays and high-sensitivity detection platforms
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Low-background fluorescence imaging
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Fluorescent biomarker tracking
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Diagnostics and proteomics applications requiring robust conjugation
Why Choose TurboTag™ 682?
TurboTag 682 combines the spectral advantages of near-infrared detection with the proven stability and efficiency of NHS ester chemistry. Optimized for reproducibility, speed, and sensitivity, the kit enables high-quality conjugation of antibodies and other proteins with minimal hands-on time. Whether for imaging, immunoassays, or analytical workflows, TurboTag 682 provides strong signal intensity, reduced background interference, and a dependable workflow for demanding research and diagnostic needs.
