Description
TurboTag 634 Protein Labeling Kit for High-Sensitivity DY-634 Conjugation
TurboTag 634 from emp BIOTECH is a complete protein labeling and purification kit for covalent conjugation of antibodies and other proteins ≥ 25 kDa using the far-red fluorophore DY-634. Leveraging NHS ester chemistry, the dye forms a stable amide linkage with primary amines on proteins, resulting in highly stable and fluorescent conjugates.
The kit contains 50 nmol of reactive dye per vial, sufficient to label up to 15 nmol of protein. TurboTag 634 conjugates exhibit strong fluorescence with excitation/emission maxima at 635 nm and 658 nm, making them ideal for multiplex detection, flow cytometry, and imaging applications. Optimized protocols for labeling both 1 mg and 100 µg of IgG are included for rapid startup.
Performance Highlights
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Effective labeling of proteins ≥ 25 kDa, especially monoclonal and polyclonal IgG
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Excitation/emission maxima at 635 nm / 658 nm for far-red detection
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Stable, covalent labeling via NHS ester–primary amine chemistry
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Pre-optimized for 1 mg and 100 µg IgG protocols
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High brightness and low background in fluorescence assays
Properties
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Molecular Weight: 1066.10
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Empirical Formula: C₄₂H₅₀N₃O₁₇S₄Na₃
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Absorption [λmax]: 635 ± 3 nm
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Emission [λmax]: 658 ± 3 nm
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Extinction Coefficient (ε): 200,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide bond)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and protein probes
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Far-red signal detection in flow cytometry and microscopy
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Imaging and immunoassays
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Multiplex fluorescence analysis
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Protein tracking and quantification in biological samples
Why Choose TurboTag™ 634?
TurboTag 634 delivers precise and high-yield conjugation with DY-634, enabling sensitive detection across far-red fluorescence platforms. Its robust NHS ester chemistry ensures reliable covalent labeling under mild conditions, while its spectral profile supports multiplexed fluorescence applications. Optimized protocols simplify workflows, making TurboTag 634 the go-to choice for researchers needing consistent, high-performance protein-dye conjugates.
