Description
TurboTag 661Q Protein Labeling Kit with Universal Quenching Dye for Silent Conjugation
TurboTag 661Q from emp BIOTECH is a specialized protein labeling and purification kit using DYQ-661, a non-emissive quencher dye with a strong absorption maximum around 662 nm. Designed for proteins ≥ 25 kDa—particularly antibodies—TurboTag 661Q enables the formation of stable quencher-protein conjugates via NHS ester chemistry. These conjugates do not fluoresce but effectively absorb light, making them ideal for FRET, quenched fluorescent probes, or fluorescence signal suppression in diagnostic and biosensor applications.
Each kit contains 50 nmol of reactive dye, sufficient to label up to 15 nmol of protein. Optimized IgG labeling protocols for both 1 mg and 100 µg are included for a seamless workflow.
Performance Highlights
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Universal quencher dye with absorption max at 662 nm
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Silent labeling: no fluorescence emission
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NHS ester chemistry for stable covalent attachment
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Optimized for proteins ≥ 25 kDa including antibodies
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Ideal for FRET, quenching assays, and probe development
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Simple, reliable workflow with pre-optimized IgG protocols
Properties
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Molecular Weight: 867.97
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Empirical Formula: C₄₃H₄₆N₃O₁₁S₂Na
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Absorption [λmax]: 662 ± 3 nm
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Extinction Coefficient (ε): 140,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form a stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescence quenching in biosensor assays
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FRET pair design and optimization
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Diagnostic assay signal suppression
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Labeling of antibodies for silent detection formats
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Real-time monitoring of binding or cleavage events
Why Choose TurboTag™ 661Q?
TurboTag 661Q delivers a high-performance, silent labeling solution for fluorescence quenching applications. Featuring DYQ-661, a highly absorptive quencher dye, it supports advanced assay design without background signal interference. The stable NHS ester linkage ensures reproducible conjugation to proteins, and the kit’s plug-and-play format saves time and increases reproducibility—especially for IgG-based workflows.
