Description
TurboTag 635 Protein Labeling Kit for High-Yield DY-633 Conjugation
TurboTag 635 from emp BIOTECH is a complete protein labeling and purification kit engineered for covalent conjugation of antibodies and other proteins ≥ 25 kDa using the far-red fluorophore DY-633. The dye’s reactive NHS ester group ensures stable amide bond formation with primary amines on the target protein, delivering robust, high-quality fluorescent conjugates.
Each vial contains 50 nmol of reactive dye, allowing labeling of up to 15 nmol of protein. The resulting conjugates feature excitation and emission maxima at 637 nm and 657 nm, respectively—ideal for far-red fluorescence imaging, multiplexing, and flow cytometry applications. The kit includes pre-optimized protocols for labeling both 1 mg and 100 µg of IgG.
Performance Highlights
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High-efficiency labeling of proteins ≥ 25 kDa, especially antibodies
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Strong far-red fluorescence at 637 nm (excitation) / 657 nm (emission)
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Stable NHS ester chemistry enables durable amide linkage formation
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Supports pre-optimized protocols for 1 mg and 100 µg IgG
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Ideal for multiplexing and background-reduced detection workflows
Properties
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Molecular Weight: 847.98
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Empirical Formula: C₄₁H₅₀N₃O₁₁S₂Na
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Absorption [λmax]: 637 ± 3 nm
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Emission [λmax]: 657 ± 3 nm
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Extinction Coefficient (ε): 200,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Antibody and protein labeling for fluorescence detection
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Flow cytometry and fluorescence microscopy
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Multiplex imaging and protein tracking
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Immunoassay development and diagnostic labeling
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High-sensitivity protein-based detection assays
Why Choose TurboTag™ 635?
TurboTag 635 delivers efficient covalent labeling with DY-633 fluorophore, offering bright, stable far-red signal performance under a range of conditions. Its compatibility with antibody formats and streamlined labeling protocols make it an ideal tool for researchers requiring consistency, sensitivity, and versatility in fluorescence-based applications.
