Description
TurboTag 481XL Protein Labeling Kit for Large Stokes Shift DY-481XL Conjugation
TurboTag 481XL from emp BIOTECH is a complete labeling and purification kit designed for the covalent conjugation of proteins with DY-481XL, a fluorophore known for its large Stokes shift. The kit is ideal for labeling proteins ≥ 25 kDa—particularly antibodies—using a reactive NHS ester of DY-481XL. The resulting dye-protein conjugates exhibit excitation at 515 nm and emission at 650 nm, providing enhanced signal separation in multi-color fluorescence applications.
Each vial contains 50 nmol of reactive dye, sufficient to label up to 15 nmol of protein. TurboTag 481XL ensures a stable, covalent amide linkage and is compatible with pre-optimized protocols for IgG labeling (1 mg or 100 µg). This makes it ideal for sensitive imaging, detection, and analytical workflows.
Performance Highlights
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Efficient labeling of proteins ≥ 25 kDa, especially monoclonal antibodies
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Large Stokes shift for reduced background and high signal clarity
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Excitation/emission at 515 nm and 650 nm for red-channel detection
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NHS ester chemistry ensures stable covalent attachment
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Each 50 nmol vial labels up to 15 nmol of protein
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Optimized protocols available for 1 mg or 100 µg IgG
Properties
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Molecular Weight: 727.75
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Empirical Formula: C₃₁H₃₄N₃O₁₂S₂Na
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Absorption [λmax]: 515 ± 3 nm
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Emission [λmax]: 650 ± 3 nm
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Extinction Coefficient (ε): 50,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and proteins
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High-resolution imaging and multi-color fluorescence
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Protein detection in immunoassays and analytical workflows
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Signal amplification and biomarker tracking in complex samples
Why Choose TurboTag™ 481XL?
TurboTag 481XL offers the performance advantages of a large Stokes shift fluorophore for precise, low-background protein labeling. Designed for proteins ≥ 25 kDa, the DY-481XL dye ensures excellent fluorescent signal separation and is covalently attached via NHS ester chemistry. Whether you are labeling IgG for diagnostic assays or developing multiplexed detection platforms, TurboTag 481XL delivers consistent, high-quality results with minimal effort.
