Description
TurboTag 554 Protein Labeling Kit for Bright DY-554 Conjugation
TurboTag 554 from emp BIOTECH is a comprehensive labeling and purification kit designed for covalent conjugation of proteins with the DY-554 fluorophore. This kit enables efficient labeling of proteins ≥ 25 kDa—especially antibodies—using a highly reactive NHS ester of DY-554 dye. The result is a stable amide linkage between the dye and primary amines on the protein, forming robust fluorescent conjugates.
TurboTag 554 conjugates emit a bright fluorescent signal with excitation at 551 nm and emission at 572 nm, ideal for applications in immunoassays, fluorescence imaging, and diagnostic workflows. Each 50 nmol dye vial is sufficient for labeling up to 15 nmol of protein, and pre-optimized protocols are available for both 1 mg and 100 µg of IgG.
Performance Highlights
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Effective fluorescent labeling of proteins ≥ 25 kDa, including IgG
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Strong fluorescence signal (excitation/emission at 551/572 nm)
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NHS ester-based covalent bonding ensures stability and reproducibility
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Compatible with standard protocols for IgG and monoclonal antibodies
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Streamlined labeling and purification in a single kit
Properties
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Molecular Weight: 677.16
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Empirical Formula: C₃₅H₃₇N₄O₈Cl
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Absorption [λmax]: 551 ± 3 nm
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Emission [λmax]: 572 ± 3 nm
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Extinction Coefficient (ε): 100,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol of protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and other proteins
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Protein detection in immunoassays and diagnostic tests
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Fluorescence microscopy and imaging
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Biomarker and molecular interaction studies
Why Choose TurboTag™ 554?
TurboTag 554 offers a powerful combination of brightness, stability, and labeling efficiency through DY-554 chemistry. Its NHS ester reactivity ensures durable protein-dye conjugates, while its high extinction coefficient delivers strong signals in fluorescence-based applications. Designed for researchers working with proteins ≥ 25 kDa, TurboTag 554 simplifies conjugation with ready-to-use reagents and proven IgG labeling protocols, enabling reliable results with minimal optimization.
