Description

Dicarboxymethylene Blue – Non-Activated Redox Marker for Electrochemical and Photosensitizer Applications

Dicarboxymethylene Blue (DCMB) is a non-activated methylene blue-based redox marker, engineered for the electro-active labeling of biomolecules. Featuring two carboxylic acid functional groups, DCMB is designed for covalent attachment to proteins and alkylamino-modified nucleic acids. Its excellent redox performance and singlet oxygen generation capacity make it highly suitable for both electrochemical labeling and photosensitizer applications.

Performance Highlights

• Dual carboxylic acid groups for increased coupling flexibility

• Validated redox functionality with a formal electrode potential of –0.216 V vs. Ag/AgCl (1 M KCl)

• High absorption and strong singlet oxygen generation

• Well-suited for both biosensor development and photodynamic uses

Properties

• Molecular Weight: 527.98

• Empirical Formula: C₂₂H₂₆N₃O₈SCl

• Absorption [nm]: 669 ± 3

• Emission [nm]: 686 ± 3

• ε [M⁻¹ cm⁻¹]: >53,000

• Buffer System for Fluorescence Readings: TE Buffer, pH 7.6

• Solubility: Acetonitrile

• Appearance (colour): dark blue

• Appearance (form): solid (powder)

• Storage: –20 °C. Keep dry. Protect from light, especially in solution

• Reactive Moiety: free carboxylic acid

Application

Biosensor measurements and electrochemical or photosensitizer labeling of proteins and oligonucleotides.