Description
5/6-TAMRA – Non-Activated Rhodamine Dye for Bright and Stable Biomolecule Labeling
5/6-Carboxytetramethylrhodamine (5/6-TAMRA) is a non-activated red fluorescent dye used for covalent labeling of proteins and alkylamino-modified nucleic acids. Although its quantum yield is lower than that of fluorescein, TAMRA conjugates frequently appear brighter due to their enhanced excitation by mercury-arc lamp (546 nm) or green He-Ne laser (543 nm) lines and their superior photostability. This makes 5/6-TAMRA an excellent choice for fluorescence microscopy and other imaging techniques where brightness and durability are essential.
Performance Highlights
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Non-activated rhodamine dye for bioconjugation
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Excitation/emission maxima: 548 nm / 570 nm
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Excellent compatibility with 543 nm and 546 nm laser lines
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Higher photostability than fluorescein
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Mixed isomers of 5- and 6-carboxytetramethylrhodamine
Properties
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Molecular Weight: 466.9
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Empirical Formula: C₂₅H₂₃ClN₂O₅
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CAS Number: 98181-63-6
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Absorption [λmax]: 548 ± 3 nm
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Emission [λmax]: 570 ± 3 nm
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Extinction Coefficient (ε): >78,000 M⁻¹·cm⁻¹
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Reactive Moiety: Free carboxylic acid
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Solubility: Methanol, DMF, DMSO
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Buffer System for Fluorescence Readings: PBS, pH 7.4
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Appearance (form/color): Violet solid (powder)
Application
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Bioconjugation of proteins and oligonucleotides
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Fluorescence microscopy
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Imaging using mercury-arc or green He-Ne lasers
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Labeling for detection assays and molecular diagnostics
