Description
High-efficiency desalting and purification of nucleic acids, proteins, and nanoparticles
CentriPure 50 – Z50M from emp BIOTECH offers fast and effective purification of biomolecules in a user-friendly, gravity-driven format. Optimized for 5 mL sample volumes, this ready-to-use column employs Zetadex-50, a cross-linked beaded dextran resin with a molecular weight cut-off (MWCO) of 25 kDa for proteins, 20 bases for nucleic acids, and >4 nm for nanoparticles.
This gel filtration column enables simultaneous desalting and purification of antibodies, enzymes, oligonucleotides, and spheroidal nanoparticles. It efficiently removes small molecules such as salts, dyes, biotin, ammonia, and haptens while maintaining high sample recovery and integrity. Ideal for buffer exchange, sample preparation, and post-synthesis cleanup, CentriPure 50 – Z50M is a robust alternative to dialysis or ultrafiltration (TFF).
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-50 (50–150 µm cross-linked beaded dextran)
- Sample Volume: 5 mL
- Elution Volume: ~7 mL
- Format: Individual gravity column
- Compatibility: Manual workflows and lab rack systems
Applications
- Desalting of DNA/RNA and oligonucleotides (>20 bp)
- Purification of antibodies and proteins (>25 kDa)
- Buffer exchange for nanoparticles (>4 nm)
- Sample prep for downstream analytics
Why Choose CentriPure 50 – Z50M?
Designed for convenience, consistency, and speed, this column delivers reliable separation without dilution or loss of sensitive biomolecules. Its prehydrated format and compatibility with lab racks make it an excellent choice for molecular biology, life sciences, and analytical chemistry labs requiring precise, small-scale purification.
