Description
Mid-scale gel filtration for high-performance desalting and buffer exchange
The CentriPure 25 – Z50M from emp BIOTECH delivers efficient, mid-volume purification and desalting of biomolecules in a single, gravity-driven step. Each column is packed with Zetadex-50, a cross-linked dextran-based size exclusion resin optimized for removing salts, dyes, biotin, ammonia, and haptens from larger macromolecules. Ideal for proteins ≥25 kDa, nucleic acids >20 bp, and nanoparticles >4 nm, this format provides clean, ready-to-use samples with minimal dilution.
With its 2.5 mL sample capacity, this column bridges the gap between small-scale and preparative workflows, offering excellent performance in applications such as DNA/RNA cleanup, antibody desalting, buffer exchange, and protein purification. The gravity-based format enables simple operation—no centrifuge, pump, or vacuum required.
Performance Highlights
- >99.99% removal of small molecules
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- Processing time under 15 minutes
- No need for centrifugation or vacuum
Specifications
- Resin: Zetadex-50 (50–150 µm particle size)
- Sample volume: 2.5 mL
- Elution volume: ~3.5 mL
- Format: Single-use, hydrated column
Applications
- DNA/RNA desalting
- Protein purification
- Buffer exchange
- Oligonucleotide cleanup
Why Choose CentriPure 25 – Z50M?
CentriPure 25 – Z50M offers fast, reliable purification performance at a mid-scale volume. With its automation-free protocol, exceptional reproducibility, and pre-filled ready-to-use format, it is an attractive alternative to time-consuming dialysis or complex TFF systems—perfect for labs that value speed, purity, and simplicity in biomolecule processing.
