Description
High-Throughput Gel Filtration for Desalting and Buffer Exchange in 96-Well Format
CentriPure 96 Gel Filtration Column Array 500 – Z25M from emp BIOTECH is a high-efficiency solution for simultaneous purification and desalting of 96 samples in a microplate-compatible format. Each column in the array is pre-packed with Zetadex-25 Medium, a beaded composite dextran resin ideal for separating proteins, nucleic acids, or nanoparticles from small contaminants such as salts, dyes, and low molecular weight reactants.
Designed for automated workflows, this 96-column format enables rapid buffer exchange or removal of small molecules with sample volumes of 500 µL per column. The optimized pore size of Zetadex-25 excludes biomolecules >5 kDa or nucleic acids >10 bp, ensuring effective separation and >85% recovery in less than 15 minutes without centrifugation.
Performance Highlights
- Parallel processing of 96 samples with sample volumes up to 500 µL
- Effective desalting and buffer exchange for proteins >5 kDa and nucleic acids >10 bp
- Fast processing: Sample purification in <15 minutes
- High yield: >85% recovery of target biomolecules
- Excellent removal of small molecules: >99.99% efficiency for salts and dyes
- Final elution volume: ~800 µL per sample
- Automated system compatible: ANSI/SLAS microplate footprint
Specifications
- Column format: 96-well plate with individual columns
- Solid phase: Zetadex-25 Medium, swollen in deionized water
- Average bead size: 50–150 µm
- Sample volume: 500 µL per column
- Elution volume: ~800 µL
- pH stability: 2–13
- Preservative: ProClin® 150
- Max backpressure: Not intended for use under pressure; gravity flow or light manifold only
Applications
- Desalting of protein and nucleic acid samples
- Buffer exchange for biomolecule workflows
- High-throughput removal of small molecules
- Sample preparation for proteomics, genomics, and nanoparticle studies
Why Choose CentriPure 96 – Z25M?
CentriPure 96 – Z25M offers unmatched ease and throughput for parallel desalting of biomolecule samples. With a simple workflow, high recovery, and microplate compatibility, it integrates seamlessly into automated platforms and accelerates sample prep efficiency. The high-resolution performance of Zetadex-25 ensures consistent separation, making it an ideal choice for researchers in molecular biology, bioprocessing, and analytical sciences.
