Description
High-Volume Gravity-Flow Desalting with CentriPure 1500 – Z25M
CentriPure 1500 – Z25M Columns are designed for efficient purification and desalting of proteins and nucleic acids in high-volume applications. These gravity-flow gel filtration columns are ideal for separating large biomolecules (such as antibodies, enzymes, or long nucleic acids) from smaller contaminants like salts, dyes, biotin, and other low molecular weight compounds. Their high recovery rate and >99.99% removal efficiency make them a powerful alternative to time-consuming dialysis or TFF.
The columns are packed with Zetadex-25 Medium, a cross-linked beaded dextran matrix developed by emp BIOTECH, optimized for rapid and reproducible separations. Designed to process up to 150 mL of sample in under 15 minutes, the column ensures minimal dilution and high throughput with consistent results across applications.
Performance Highlights
- Efficient removal of small molecules with >99.99% desalting efficiency
- 85% recovery of proteins and nucleic acids
- Sample volumes up to 150 mL processed in <15 minutes
- Rapid, gravity-based purification with no need for centrifugation or vacuum
- Ideal for antibodies, enzymes, DNA, RNA, and nanoparticles ≥ 2 nm
- Fractionation range: ~5 kDa for proteins / ~10 base pairs for nucleic acids
Specifications
- Matrix: Zetadex-25 Medium
- Bead size: 50–150 µm
- Sample volume: up to 150 mL
- Bed volume: 195 mL
- Recommended buffer volume: 195 mL
- Dilution factor: ~1.5×
- Flow type: Gravity
- Processing time: <15 minutes
- Format: Ready-to-use, single-use column
- Manufactured by: emp BIOTECH, Berlin-Buch
Applications
- Desalting of proteins, nucleic acids, and nanoparticles
- Buffer exchange
- Removal of dyes, salts, haptens, and unreacted small molecules
- High-throughput sample preparation for downstream processing
Why Choose CentriPure 1500 – Z25M?
CentriPure 1500 – Z25M is the ideal solution for laboratories and production environments requiring fast, scalable purification of high-volume samples. The column’s gravity-based design eliminates the need for specialized equipment while delivering unmatched desalting efficiency and recovery. Its compatibility with a broad range of biomolecules and buffers ensures flexible integration into existing protocols. Whether you’re performing preparative cleanup or downstream processing, this column provides dependable, high-yield results with exceptional ease of use.
