Description
High-throughput desalting and purification of nucleic acids, proteins, and nanoparticles at large scale
The CentriPure 1000 – Z25M column from emp BIOTECH is engineered for high-volume, gravity-driven purification and desalting of up to 100 mL of biological samples. Packed with Zetadex-25, a cross-linked beaded dextran resin, this column efficiently removes low-molecular-weight contaminants such as salts, dyes, biotin, ammonia, and haptens from larger biomolecules including proteins (>5 kDa), nucleic acids (>10 bp), and nanoparticles (>2 nm). Its optimized bed volume ensures exceptional reproducibility and high recovery rates in DNA, RNA, protein, and nanoparticle workflows.
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-25 (50–150 µm cross-linked dextran)
- Sample Volume: 100 mL
- Flow Rate: <20 min for 100 mL water
- Salt Removal Efficiency: <1000 µS/cm (from 0.8 M NaCl input)
- Recovery: >85% Dextran Blue recovery
- Format: Single-use gravity column
- Storage: Ambient, protected from light
- Preservative: ProClin® 150
Applications
- Large-scale DNA/RNA desalting
- Antibody and protein purification
- Nanoparticle buffer exchange
- Preparation of biomolecules for downstream processing or analysis
Why Choose CentriPure 1000 – Z25M?
This high-capacity format delivers speed, scalability, and consistent results without the need for complex equipment or extended processing times. Whether you’re performing buffer exchange, post-synthesis cleanup, or sample prep for analysis, CentriPure 1000 – Z25M offers a reliable alternative to time-consuming dialysis and ultrafiltration systems.
