Description
High-efficiency desalting and purification for mid-scale biomolecule preparation
CentriPure 200 – Z25M from emp BIOTECH is a prehydrated gel filtration column optimized for rapid purification, desalting, and buffer exchange of 20 mL samples containing nucleic acids, proteins, antibodies, or nanoparticles. This gravity-flow column provides a convenient and scalable alternative to dialysis and tangential flow filtration (TFF), delivering fast and reproducible separation of low-molecular-weight impurities—including salts, dyes, biotin, haptens, and ammonia—from biomolecules larger than 5 kDa, oligonucleotides longer than 10 bp, and nanoparticles above 2 nm.
Packed with Zetadex-25, a cross-linked beaded dextran size-exclusion resin with a 50–150 µm particle size range, CentriPure 200 enables high-resolution separation in under 15 minutes without the need for centrifugation or vacuum. Its optimized bed volume and column geometry ensure consistent performance and excellent yield for a wide range of molecular biology and bioprocessing workflows.
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-25 (cross-linked beaded dextran, 50–150 µm)
- Sample volume: 20 mL
- Flow rate: <6 minutes (20 mL water)
- Salt removal: <1000 µS/cm (from 0.8 M NaCl load)
- Recovery yield: >85% (Dextran Blue test)
- pH stability: 2–13
- Preservative: ProClin® 150
- Storage: Ambient, protected from light
- Format: Individual column for gravity flow
Applications
- DNA/RNA desalting and buffer exchange
- Protein and antibody purification
- Nanoparticle cleanup and sample prep
- Post-synthesis cleanup for nucleic acids
Why Choose CentriPure 200 – Z25M?
CentriPure 200 – Z25M is ideal for researchers requiring efficient, ready-to-use purification columns for mid-scale sample processing. With its high removal efficiency, reliable recovery, and compatibility with a variety of biomolecules, it simplifies lab workflows and improves downstream performance.
