Description
High-yield desalting and purification for proteins, nucleic acids, and nanoparticles
The CentriPure 100 – Z25M from emp BIOTECH is a high-performance gel filtration column designed for efficient desalting, buffer exchange, and purification of biomolecules. Optimized for sample volumes of 10 mL, this ready-to-use column employs Zetadex-25, a cross-linked dextran-based size-exclusion resin that effectively removes small molecules such as salts, dyes, biotin, ammonia, and haptens from larger macromolecules.
Ideal for biomolecules including nucleic acids longer than 10 bp, proteins larger than 5 kDa, and nanoparticles exceeding 2 nm, this gravity-driven column enables simultaneous purification and buffer exchange in less than 15 minutes—making it a fast and scalable alternative to dialysis or ultrafiltration (TFF).
Performance Highlights
- >99.99% removal of small molecule contaminants
- >85% recovery of nucleic acids and proteins
- 1.5-fold dilution typical
- <15-minute processing time
- No centrifugation or vacuum required
Specifications
- Resin: Zetadex-25 (50–150 µm particle size)
- Sample volume: 10 mL
- Column bed: Hydrated and ready to use
- pH stability: 2–13
- Preservative: ProClin® 150
- Storage/Shipping: Ambient
Applications
- DNA/RNA desalting
- Protein purification
- Buffer exchange
- Nanoparticle cleanup
Why Choose CentriPure?
Backed by ISO-certified quality, CentriPure 100 – Z25M offers robust performance, reproducibility, and compatibility with a wide range of purification and cleanup workflows in molecular biology, proteomics, and bioprocessing research. Its rapid operation and high-yield recovery make it a reliable tool for any lab requiring mid-scale purification with minimal hands-on time.
