Description
Scalable Desalting and Purification of Biomolecules in 5 mL Format
The CentriPure 50 – Z25M column from emp BIOTECH delivers fast and efficient gravity-driven purification and desalting of biomolecules in 5 mL sample volumes. Engineered for proteins larger than 5 kDa, nucleic acids longer than 10 bp, and spheroidal nanoparticles >2 nm, this column removes small molecular contaminants such as salts, dyes, haptens, ammonia, and biotin in a single step—no centrifugation or vacuum required.
Each column is packed with Zetadex-25, a cross-linked beaded dextran resin with an effective exclusion limit of ~5 kDa. Molecules larger than the gel pores elute quickly in the void volume, while smaller contaminants are retained, enabling >99.99% removal efficiency with >85% recovery of target molecules and ~1.5-fold dilution. The prehydrated, ready-to-use format supports rapid sample preparation and reproducible results in under 15 minutes.
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-25 (50–150 µm cross-linked beaded dextran)
- Sample Volume: 5.0 mL
- Format: Individual column
- Preservative: ProClin® 150
- pH Stability: 2–13
- Storage: Ambient
Applications
- Desalting of proteins and oligonucleotides
- Buffer exchange for antibodies and enzymes
- Post-synthesis cleanup
- Nanoparticle purification
Why Choose CentriPure?
CentriPure 50 – Z25M is a cost-effective and automation-independent alternative to dialysis and ultrafiltration (TFF), ideal for life science, molecular biology, and bioprocessing labs seeking fast, reproducible, and high-yield purification at mid-scale volumes.
