Description
High-efficiency desalting and purification of nucleic acids, proteins, and nanoparticles at small scale
The CentriPure 5 – Z25M column from emp BIOTECH is a compact and powerful tool for rapid, gravity-driven desalting and purification of nucleic acids, proteins, antibodies, and nanoparticles. Designed for 0.5 mL sample volumes, this prehydrated gel filtration column ensures the simultaneous removal of low molecular weight contaminants such as salts, dyes, biotin, haptens, and ammonia while preserving the integrity and yield of target biomolecules.Packed with Zetadex-25, a cross-linked beaded dextran size-exclusion resin, CentriPure 5 offers exceptional removal efficiency (>99.99%) and excellent recovery rates (>85%) for molecules above 5 kDa, oligonucleotides >10 bp, and nanoparticles >2 nm. It is ideal for post-synthesis cleanup, buffer exchange, and sample preparation workflows across life science, molecular biology, and analytical labs.
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-25 (50–150 µm particle size)
- Resin: Zetadex-25 (50–150 µm cross-linked beaded dextran)
- Sample Volume: 500 µL
- Format: Individual column
- Compatibility: Manual workflows or multichannel pipette processing
Applications
- DNA/RNA purification and desalting
- Antibody and protein cleanup
- Nanoparticle buffer exchange
- Preparation for downstream analysis or synthesis
Why Choose CentriPure?
CentriPure 5 – Z25M is optimized for fast, reproducible purification with gravity-based flow. It eliminates the need for time-consuming dialysis or ultrafiltration (TFF) while maintaining high yield and sample integrity. Its prehydrated format enables immediate use in clean-up and buffer exchange workflows, offering a consistent and reliable tool for researchers working with small sample volumes.
