Description
High-Efficiency Desalting and Purification for Low-Volume Biomolecular Samples
The CentriPure 2 – Z25M column from emp BIOTECH is engineered for fast and effective desalting and purification of proteins, nucleic acids, and nanoparticles in sample volumes ranging from 150 µL to 300 µL. Pre-filled with Zetadex-25, a cross-linked beaded dextran resin, this gravity-flow column removes small molecules such as salts, dyes, biotin, haptens, and ammonia while preserving sample yield and integrity.
With its rapid workflow (<15 minutes), outstanding salt removal (>99.99%), and high recovery rates (>85%), CentriPure 2 – Z25M is ideal for applications in molecular biology, life sciences, and analytical research. It provides a reliable alternative to dialysis and ultrafiltration, streamlining buffer exchange and post-synthesis cleanup.
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-25 (50–150 µm particle size)
- Sample Volume: 150–300 µL
- Format: Individual prehydrated column
- pH Stability: 2–13
- Preservative: ProClin® 150
- Compatibility: Manual workflows or multichannel pipette processing
Applications
- Desalting and buffer exchange of DNA/RNA
- Purification of proteins, enzymes, and antibodies
- Removal of small molecules from nanoparticle preparations
- Cleanup after oligo synthesis and labeling
Why Choose CentriPure?
CentriPure 2 – Z25M offers reproducible, high-efficiency purification for small sample volumes without the need for complex equipment. With pre-equilibrated convenience and compatibility with custom buffers, it supports a wide range of downstream analytical and preparative workflows.
