Description
Rapid Parallel Desalting with CentriPure 96 Replacement Columns
CentriPure 96 Gel Filtration Column Array 400 – Z25M Replacement Columns are supplied as single hydrated columns and are used as exchangeable units in the CP-0581 array system. Designed to process 96 samples in parallel, these columns provide a rapid and efficient method for removing small molecules such as salts, dyes, biotin, ammonia, haptens, and other low molecular weight contaminants from larger proteins, nucleic acids, or spheroidal nanoparticles. This simultaneous desalting and purification approach is ideal for high-throughput labs seeking fast buffer exchange or sample cleanup prior to downstream analysis.
The column resin is Zetadex-25 Medium, a cross-linked beaded dextran solid phase with particle sizes between 50–150 µm, developed by emp BIOTECH for gravity flow applications. This size-exclusion resin has a molecular weight cut-off (MWCO) of approximately 5 kDa for proteins and 10 bp for nucleic acids. Molecules larger than the gel pores pass rapidly through the void volume and elute free of small contaminants. Recovery of proteins or nucleic acids typically exceeds 85%, with small molecule removal efficiency over 99.99% and minimal dilution (1.5–2-fold).
These columns are optimized for use with automated liquid handling systems using the ANSI/SLAS microplate format and allow sample processing in under 15 minutes.
Performance Highlights
- Purifies and desalts proteins > 5 kDa and nucleic acids > 10 bp
- Suitable for spheroidal nanoparticles > 2 nm in diameter
- High throughput: 96 samples simultaneously
- High recovery rates: > 85% for proteins and oligos
- Small molecule removal: > 99.99%
- Dilution factor: ~1.5–2-fold
- Ideal for automated workflows (ANSI/SLAS format)
- Sample processing time: < 15 minutes
Specifications
- Bed volume: ~400 µL
- Sample volume: 400 µL
- Resin: Zetadex-25 Medium (cross-linked dextran)
- Bead size: 50–150 µm
- MWCO: ~5 kDa (proteins), ~10 bp (nucleic acids)
- Recovery: > 85%
- Small molecule removal: > 99.99%
- Recommended method: Gravity flow or light vacuum
- Footprint: ANSI/SLAS standard
Applications
- Desalting of protein or nucleic acid solutions
- Removal of small molecules, such as salts, dyes, and biotin
- Buffer exchange for downstream compatibility
- High-throughput processing in automation-compatible platforms
Why Choose CentriPure 96 Gel Filtration Column Array 400 – Z25M Replacement Columns?
These columns deliver unmatched desalting performance with high sample recovery and minimal dilution, making them ideal for laboratories requiring efficient, parallel processing. Designed to fit seamlessly into automated workflows, they combine rapid throughput with the precision of a size-exclusion matrix tailored for biomolecular purification. Whether for sample cleanup or buffer exchange, CentriPure 96 Replacement Columns are the smart, scalable solution for modern molecular biology and protein analysis workflows.
