Description
High-Throughput Desalting and Buffer Exchange for 96 Samples in a Convenient Replacement Format
CentriPure 96 Gel Filtration Column Array 500 Replacement Columns are designed for high-throughput purification and desalting of nucleic acids, proteins, and nanoparticles. These individually packed, pre-hydrated columns are ideal for replacing single columns in the CP-0582 array system, offering maximum flexibility in automated workflows. The columns efficiently separate larger biomolecules—such as antibodies, enzymes, and long oligonucleotides—from low molecular weight contaminants including salts, dyes, biotin, and haptens. With sample volumes up to 500 µL and a standard ANSI/SLAS microplate footprint, these columns enable rapid, reproducible processing by gravity or gentle vacuum.
Packed with Zetadex-25 Medium, a cross-linked beaded dextran matrix manufactured by emp BIOTECH, the columns provide high-resolution separation and robust chemical stability. Molecules larger than the exclusion limit (approximately 5 kDa for proteins or 10 bp for nucleic acids) are rapidly eluted in the void volume, free of small molecule impurities. With a typical recovery rate exceeding 85% and small molecule removal efficiency >99.99%, CentriPure 96 Replacement Columns ensure clean and undiluted results in under 15 minutes.
Performance Highlights
- Processes up to 96 samples simultaneously (500 µL each)
- Compatible with gravity flow and light vacuum
- Sample recovery >85%; small molecule removal >99.99%
Specifications
- Matrix: Zetadex-25 Medium (cross-linked beaded dextran)
- Bead size: 50–150 µm
- Exclusion limit: ~5 kDa for proteins, ~10 bp for nucleic acids
- Sample volume: 500 µL
- Processing time: <15 minutes
- Storage: Pre-hydrated; store at room temperature
- Footprint: Standard 96-well ANSI/SLAS microplate
Applications
- Desalting of antibodies, enzymes, proteins
- Buffer exchange for nucleic acids and nanoparticles
- High-throughput cleanup of labeled biomolecules and synthesis products
Why Choose CentriPure 96 Replacement Columns?
CentriPure 96 Replacement Columns combine the efficiency of high-throughput processing with the precision of gravity-based size-exclusion chromatography. Their compatibility with automated systems, consistent recovery rates, and flexible handling make them the ideal solution for routine purification and buffer exchange in demanding lab environments.
