Description
Flexible High Efficiency Desalting and Purification for Evaluation and Method Development
The CentriPure Trial Pack from emp BIOTECH offers an easy entry point for testing gravity-flow gel filtration across multiple sample volumes. Each pack contains one prehydrated Zetadex-25–based column in five different sizes: 200 µL, 500 µL, 1.0 mL, 2.5 mL, and 5.0 mL. These columns are engineered for rapid and reliable desalting, buffer exchange, and small molecule removal from nucleic acids, proteins, and antibodies—all in less than 15 minutes.
The proprietary Zetadex-25 resin is a cross-linked beaded dextran size-exclusion matrix with an effective exclusion limit of ~5 kDa. It enables >99.99% removal of small molecules such as salts, dyes, biotin, haptens, and buffer components, while ensuring high recovery (>85%) of oligonucleotides (>10 bp) and proteins (>5 kDa). This pack is perfect for labs evaluating multiple sample volumes or developing scalable purification protocols.
Performance Highlights
- >99.99% removal of low-molecular-weight contaminants
- >85% recovery of nucleic acids and proteins
- ~1.5-fold sample dilution
- <15-minute processing time
- Gravity flow operation – no centrifugation or vacuum needed
Specifications
- Resin: Zetadex-25 (cross-linked dextran, 50–150 µm)
- Sample Volumes: 200 µL, 500 µL, 1.0 mL, 2.5 mL, 5.0 mL
- Format: Individual gravity-flow columns
- Compatibility: Manual use or multichannel pipettes
Applications
- Protein desalting and buffer exchange
- Oligonucleotide purification post-synthesis
- Removal of dyes, salts, biotin, and haptens
- Small-scale method development and sample comparison
Why Choose CentriPure?
Whether you’re optimizing workflows, comparing recovery across different volumes, or exploring new purification needs, the CentriPure Trial Pack gives you a flexible and efficient toolset. Designed to eliminate the time and complexity of dialysis or TFF, these gravity columns deliver consistent, reproducible results across a variety of biomolecules and applications.
