Description
High-throughput desalting and cleanup for DNA sequencing workflows
The CentriPure 96 – 800SD25 from emp BIOTECH is a ready-to-use 96-well gel filtration plate designed for the efficient removal of small molecular contaminants in automated or manual workflows. Filled with Zetadex-25SF, a cross-linked beaded dextran matrix, this plate enables rapid purification of oligonucleotides and sequencing reaction products, including the removal of excess DyeDeoxy™ and BigDye™ terminators.
Each well contains a uniform hydrated gel bed (400 µL) within an 800 µL total volume and is supported by a high-density PE filter membrane. The short drip directors ensure compatibility with standard lab equipment and robotic systems. Samples ranging from 10 to 50 µL are processed within 15 minutes using a centrifuge, delivering highly consistent signal intensities and clean reads starting from the first base.
Performance Highlights
- Filled with Zetadex-25SF, hydrated in sterile, deionized water
- 800 µL total well volume, suitable for 10–50 µL sample input
- Short drip directors for standard automation and manual pipetting
- >99.99% small molecule removal, including dyes and salts
- Centrifugation-based workflow with visual matrix validation
Specifications
- Matrix: Zetadex-25SF (cross-linked beaded dextran)
- pH stability: 2–13
- Storage: 4–8 °C (do not freeze)
- Well material: Sterile medical-grade polypropylene
- Membrane: Ultra-high molecular weight PE (25 µm pore size)
Applications
- Desalting of oligonucleotides (≥10 bp)
- Removal of DyeDeoxy™, BigDye™, and sequencing reagents
- Pre- and post-synthesis cleanup for nucleic acid workflows
Why Choose CentriPure 96 – 800SD25?
Designed for both manual and robotic use, CentriPure 96 – 800SD25 offers high-performance, plate-based cleanup of sequencing reactions with exceptional reproducibility, ease of use, and compatibility with standard centrifuge equipment—ideal for high-throughput labs looking to streamline nucleic acid purification.
