Description
High-throughput purification of sequencing reactions and oligonucleotides
The CentriPure 96 – 400 µL plate from emp BIOTECH is a high-performance gel filtration plate designed for the simultaneous cleanup of 96 DNA sequencing reactions. Filled with Zetadex-50 and equipped with long drip directors, this sterile, ready-to-use 96-well plate removes unincorporated DyeDeoxy and BigDye™ terminators, salts, and low-molecular-weight impurities. It delivers high recovery and uniform read signal intensity starting from the first base across all wells.
Each well has a 400 µL volume and contains a ~320 µL hydrated gel bed supported by an individual 25 µm PE membrane. The plates are optimized for gravity or centrifugation-based workflows and compatible with automated liquid handling systems, enabling efficient purification in under 15 minutes.
Performance Highlights
- Simultaneous cleanup of 96 sequencing reactions
- High removal efficiency of excess terminators and salts
- Uniform signal intensity across all wells
- Compatible with robotic systems or multichannel pipettes
- Processing time: <15 minutes
Specifications
- Gel Matrix: Zetadex-50
- Format: 96-well plate with long drip directors
- Well Volume: 400 µL
- Gel Bed Volume: ~320 µL
- Membrane: 25 µm ultra-high MW PE
- Mode of Operation: Centrifugation (recommended 1000 × g for 3 min)
Applications
- Oligonucleotide purification
- Removal of DyeDeoxy and BigDye™ terminators
- Desalting prior to sequencing or downstream analysis
Why Choose CentriPure 96 – 400 µL, Long Drip Directors?
For researchers seeking a consistent and reliable solution for sequencing reaction cleanup, CentriPure 96 offers fast processing, minimal cross-contamination, and high-quality sequencing results—all in a format compatible with automated systems.
