Description
High-Throughput Dye Terminator Cleanup in 384-Well Format
The CentriPure 384 from emp BIOTECH is a high-efficiency gel filtration plate engineered for the simultaneous cleanup of 384 DNA sequencing reactions. Packed with Zetadex-50SF, a superfine cross-linked dextran resin, this prehydrated 384-well plate rapidly removes excess DyeDeoxy and BigDye terminators from Sanger sequencing reactions, ensuring sharp signal starts and uniform intensity across all wells.
Designed for processing sample volumes of 2–8 µL, each well provides consistent gel bed heights supported by a 25 µm membrane and accommodates both manual and automated protocols. The system integrates seamlessly into high-throughput workflows, with a total cleanup time of less than 15 minutes.
Performance Highlights
- Efficient dye terminator removal for 384 reactions
- Optimized for sample volumes of 2–8 µL
- Pre-filled with Zetadex-50SF for high-resolution separation
- No preservatives, salt, or buffer—hydrated in pure water
- Uniform gel bed height for robotic compatibility
- Centrifugation-based protocol with visual gel bed inspection
Specifications
- Resin: Zetadex-50SF (Superfine cross-linked dextran)
- Well Volume: 100 µL
- Bed Volume: ~75 µL
- Format: 384-well medical-grade polypropylene plate
- Filter: 25 µm UHMW PE membrane
- Storage: 4–8 °C
Applications
- Dye terminator removal in DNA sequencing
- Oligonucleotide desalting and purification
- Sample preparation for high-throughput sequencing workflows
Why Choose CentriPure 384?
Achieve fast, reliable, and uniform sequencing cleanup across 384 samples with CentriPure 384—ready-to-use, automation-compatible, and precision-packed for consistent, high-quality results in just minutes.
