Description
High-throughput Dye Terminator Removal and Desalting in 96-Well Format
The CentriPure 96 – 800 µL from emp BIOTECH is a ready-to-use gel filtration plate designed for the rapid and reliable removal of excess dye terminators and small molecules from DNA sequencing reactions and oligonucleotide samples. Packed with Zetadex-50SF, each of the 96 wells supports sample purification with high reproducibility, delivering clean reads with uniform signal intensity from the first base.
Engineered for throughput and consistency, each well features a hydrophilic 400 µL gel bed supported by a 25 µm PE membrane, enclosed in a sterile medical-grade polypropylene plate. With a sample volume range of 5–30 µL, all 96 reactions can be processed simultaneously using a standard centrifuge and collection plate, without need for vacuum or additional instrumentation.
Performance Highlights
- Effective removal of dye terminators and small molecules
- Consistent read quality across all 96 wells
- <15-minute spin protocol for complete plate
- Precision-filled with Zetadex-50SF for high reproducibility
- Ready-to-use, hydrated with pure water – no preservatives
Specifications
- Resin: Zetadex-50SF (20–50 µm cross-linked beaded dextran)
- Well Volume: 800 µL
- Gel Bed Volume: ~400 µL
- Sample Volume: 5–30 µL per well
- Membrane: 25 µm PE
- Plate Format: ANSI/SLAS 96-well, short drip directors
- Preservative: None
- Storage: 2–8 °C
Applications
- Dye terminator cleanup in Sanger sequencing
- Desalting of oligonucleotides and nucleic acid samples
- Buffer exchange for low-volume biomolecular preparations
Why Choose CentriPure 96 – 800 µL?
CentriPure 96 ensures fast, reproducible purification for DNA sequencing workflows—ideal for labs seeking reliable performance in high-throughput formats without the complexity of vacuum systems or robotics.
