Description
DCMB-SE: Redox-Active NHS Ester for Electrochemical and Photodynamic Labeling
Dicarboxymethylene Blue NHS ester (DCMB-SE) is an amine-reactive redox dye tailored for covalent labeling of proteins and alkylamino-modified nucleic acids. Containing two activated carboxylic acid functional groups, DCMB-SE offers high absorption and excellent redox properties. Its ability to efficiently generate singlet oxygen makes it an excellent choice for electrochemical labeling as well as photosensitizer-based applications.
Performance Highlights
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Dual carboxyl functionality enables efficient and stable labeling
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Excellent redox performance with validated electrode potential
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Strong singlet oxygen generator for photodynamic applications
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Formal Electrode Potential vs. Ag/AgCl (1 M KCl): –0.216 V
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High absorptivity at 669 nm, emission at 686 nm
Properties
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Molecular Weight: 722.11
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Empirical Formula: C₃₀H₃₂N₅O₁₂SCl
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Absorption [nm]: 669 ± 3
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Emission [nm]: 686 ± 3
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ε [M⁻¹ cm⁻¹]: >53 000
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Buffer System for Fluorescence Readings: TE Buffer, pH 7.6
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Solubility: Acetonitrile
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Appearance (colour): dark blue
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Appearance (form): solid (powder)
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Storage: –20 °C. Keep dry. Protect from light, especially in solution
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Reactive Moiety: NHS ester (reacts with 1° amines to form a stable amide linkage)
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Trade Name: Dicarboxymethylene blue succinimidyl ester; Dicarboxymethylene blue NHS ester
Application
Bioconjugation, biosensor measurements, and photosensitizer-based labeling strategies
