Fast Protein Liquid Chromatography Solutions for Antibodies and Recombinant Proteins
FPLC Purification
emp BIOTECH’s FPLC-ready ZetaSep columns and SMIRF™ screening kits support high-yield purification of proteins, antibodies, and tagged biomolecules. Designed with cross-linked agarose resins and optimized for ion exchange, affinity, and hydrophobic interaction chromatography, these columns deliver excellent resolution, pressure stability, and reproducible scale-up from 1 mL to preparative workflows.
The SMIRF™ (Small-Scale Integrated Rapid FPLC) kits provide pre-configured column sets for fast method development and ligand screening across IMAC, IEX, and antibody purification formats.
The following formats are available for FPLC purification:
Ion Exchange – A widely used method based on surface charge
Affinity – For selective binding of antibodies and tagged proteins
Hydrophobic Interaction – Enables gentle purification under high-salt conditions
SMIRF™ – FPLC Screening Kit – For rapid evaluation of ligands and conditions
SMART Chromatography™ DEAE
SMART Chromatography™ column packed with Weak Anion Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ column packed with Strong Anion Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ CM Boost
SMART Chromatography™ column packed with Weak Cation Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ DEAE
SMART Chromatography™ column packed with Weak Anion Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ SP
SMART Chromatography™ column packed with Strong Cation Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ SP Boost
SMART Chromatography™ column packed with Strong Cation Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ CM
SMART Chromatography™ column packed with Weak Cation Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
SMART Chromatography™ DEAE Boost
SMART Chromatography™ column packed with Weak Anion Exchange Agarose Resin. Specifically designed for SMART Chromatography™ ion exchange separation.
Zetasept SP FF6
FPLC column packed with Strong Cation Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.
ZetaSep DEAE FF6
FPLC column packed with Weak Anion Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.
ZetaSep CM FF6
FPLC column packed with Weak Cation Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.
ZetaSep Q FF6
FPLC column packed with Strong Anion Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.
ZetaSep Ni-NTA FF6
FPLC column packed with Ni-NTA Agarose Resin. A cross-linked 6% beaded agarose IMAC resin for His-tagged protein purification.
ZetaSep NTA (metal-free) FF6
FPLC column packed with NTA Agarose Resin. A cross-linked 6% beaded agarose IMAC resin for His-tagged protein purification.
ZetaSep Protein A Elevate
FPLC column packed with NaOH-tolerant recombinant Protein A Agarose Resin. A cross-linked beaded agarose resin to purify immunoglobulins via their selective affinity to Protein A.
ZetaSep Phenyl FF6
FPLC column packed with HIC resin, derivatized with phenyl ligand. A cross-linked 6% beaded agarose resin for hydrophobic interaction chromatography purification.
ZetaSep Phenyl HC
FPLC column packed with high capacity HIC resin, derivatized with phenyl ligand. A small-sized cross-linked beaded agarose resin for hydrophobic interaction chromatography purification.
ZetaSep Butyl FF6
FPLC column packed with HIC resin, derivatized with butyl ligand. A cross-linked 6% beaded agarose resin for hydrophobic interaction chromatography purification.
SMIRF™ Immobilised Metal Affinity (IMAC) Purification, 4 x 1 mL 1 x 5 mL
Includes 1 mL ZetaSep columns packed with Zetarose Ni-NTA, Zetarose Co-NTA, Zetarose Cu-NTA and Zetarose Zn-NTA solid phases and a 5 mL Zetadex Desalting column.
SMIRF™ Ion Exchange (IEX) Purification, 4 x 1 mL 1 x 5 mL
Includes 1 mL ZetaSep columns packed with Zetarose CM, Zetarose DEAE, Zetarose Q and Zetarose SP solid phases and a 5 mL Zetadex Desalting column.
SMIRF™ Antibody Purification, 2 x 1 mL 1 x 5 mL
Contains a 1 mL base-stable (1 M NaOH) Zetarose Protein A Elevate® column, a 1 mL Zetarose Phenyl hydrophobic interaction (HIC) column and a 5 mL Zetadex Desalting column.