Affinity Chromatography Resins for Antibody and His-Tagged Protein Purification
Affinity Chromatography: Agarose-Based Resins for Selective Protein Purification
Affinity chromatography is a widely used and powerful purification technique. Its power and popularity derive from using the highly selective interaction between a target molecule and a ligand that is immobilized on a solid support. emp BIOTECH’s agarose-based resins allow for a wide range of ligands to be immobilized, giving our customers a range of options for purifying antibodies (Zetarose Protein A Elevate, Protein G Elevate) and His-tagged proteins (Zetarose NTA, with metal-free, Cu, Ni, Zn and Co options).
Depending on your target and method, other separation principles may be more suitable:
Ion Exchange Resins: For charge based separation of proteins and nucleic acids
Hydrophobic Interaction Resins: For salt-dependent separation of proteins by surface hydrophobicity
Zetarose NTA FF6
6 % Beaded agarose resin immobilized with metal-free nitrilotriacetic acid (NTA) affinity ligand.IMAC affinity resin for His-tagged protein purification.
Zetarose Ni-NTA FF6
6 % Beaded agarose resin immobilized with Ni²⁺ charged nitrilotriacetic acid (Ni-NTA).IMAC affinity resin for His-tagged protein purification.
Zetarose Protein A Elevate
Beaded agarose resin immobilized with NaOH tolerant recombinant Protein A ligand.A cross-linked affinity resin for immunoglobulin protein purification.
ZetaCell Protein A Elevate
Large diameter beaded agarose resin immobilized with NaOH-tolerant recombinant Protein A ligand.Specifically designed for SMART™ affinity chromatography of immunoglobulin proteins.
ZetaCell Ni-NTA
Large diameter beaded agarose resin immobilized with Ni²⁺ charged nitrilotriacetic acid (Ni-NTA) for the purification of His-tagged proteins. Specifically designed for SMART™ affinity chromatography of His-tagged proteins.
ZetaCell NTA
Large diameter beaded agarose resin immobilized with uncharged nitrilotriacetic acid (NTA).Specifically designed for SMART™ affinity chromatography of His-tagged proteins.