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Gravity Flow Gel Filtration for Rapid Desalting and Buffer Exchange

Dextran-Based Size Exclusion Columns and Plates for Proteins, Oligonucleotides, and Nanoparticles

CentriPure™ is emp BIOTECH’s versatile product line for desalting, buffer exchange, and purification using Zetadex™, a dextran-based size exclusion resin. Whether working with antibodies, oligonucleotides, enzymes, or nanoparticles, CentriPure™ enables the rapid and efficient removal of small molecules—such as salts, dyes, biotin, or haptens—from larger biomolecules in a single step. In general, CentriPure Gel Filtration Columns can be used for the same applications as the PD-10, the NAP-5, NAP-10 or NAP-25 columns which are manufactured by Cytiva.

The product range covers volumes from 150 µL to 150 mL and supports both manual and automated workflows. Whether you’re purifying antibodies, enzymes, oligonucleotides, or nanoparticles, CentriPure™ delivers high recovery with minimal dilution.

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Key Features and Benefits of CentriPure™ Gel Filtration Columns

CentriPure™ columns deliver fast, reproducible desalting and buffer exchange across a broad range of sample volumes and applications. All columns are pre-packed with ultrapure Zetadex™ dextran-based resin and are ready to use—no resin preparation, equilibration, or setup required.

Pre-packed and ready to use
No setup, swelling, or resin handling—just equilibrate and purify.

Gravity-flow operation
Standalone workflow with no need for centrifuges or pumps.

Two MWCO options
Zetadex-25 (5 kDa / 10 bp) and Zetadex-50 (25 kDa / 20 bp) for targeted separation.

High recovery and resolution
≥85% recovery with efficient removal of salts, dyes, biotin, and haptens.

Scalable fixed-volume formats
Available in 11 sizes from 150 µL to 150 mL.

Endotoxin-free options
Certified low-endotoxin formats for sensitive workflows.

Explore the CentriPure™ Product Formats

CentriPure™ Columns

Gravity Flow Desalting from 150 µL to 150 mL

Pre-packed, fixed-volume columns for desalting and buffer exchange without centrifugation.

  • 11 sizes available

  • Elution volume = 1.5× sample volume

  • For proteins, nucleic acids, and nanoparticles

Learn more about the CentriPure gravity columns.

ZetaSpin MINI Columns

Spin Desalting up to ≤100 µL

Sterile, conical centrifugation columns for ultrafast cleanup of small-volume samples.

  • Removes up to 99.999% salts, dyes, biotin, and haptens

  • Processing time: under 5 minutes

Learn more about the ZetaSpin MIDI columns.

ZetaSpin MIDI Columns

Centrifugation Desalting — 500 to 700 µL

Hydrated columns with integrated adapter for secure and consistent spinning.

  • Removes up to 99.99% of small molecules

  • Ideal for dye-labeled proteins and oligos

Learn more about the ZetaSpin MIDI columns.

CentriPure™ Column Arrays

96-Well High-Throughput Desalting — 300 to 500 µL

SLAS-compatible arrays for automated parallel sample cleanup.

  • For robots, liquid handlers, or vacuum manifolds

  • Process 96 samples in parallel

Learn more about the CentriPure 96-well column arrays

CentriPure™ Plates

Desalting Plates — Up to 70 µL in 96- and 384-Well Formats

Designed for high-throughput labs using small sample volumes.

  • No dilution of sample

  • Optional drip directors prevent cross-contamination

Learn more about the CentriPure desalting plates for high-throughput labs.

ZetaSep™ Desalting Columns

Post-FPLC Buffer Exchange — 200 µL to 1 mL

Pre-packed columns for inline monitoring and polishing steps.

  • Pressure compatible up to 3 bar

  • Suitable for UV-based elution tracking

Learn more about the ZetaSep desalting.

Zetadex™ Resin: Consistent, High-Performance Separation

All CentriPure™ formats are powered by Zetadex™, a proprietary, cross-linked dextran gel filtration resin.

  • Zetadex-25: MWCO ~5 kDa (proteins) / 10 bases (nucleic acids)

  • Zetadex-50: MWCO ~25 kDa (proteins) / 20 bases (nucleic acids)

Ultrapure gel and specially treated frits ensure outstanding resolution, low sample loss, and minimal cross-contamination.

Reliable Dextran-Based Alternative to Sephadex® for Gel Filtration Applications

The emp BIOTECH offering provides a like-for-like alternative to established dextran-based media such as Sephadex® and other comparable gel filtration resins—delivering equivalent performance in size-based separation, chemical stability, and application versatility.

Protein and Antibody Purification

Removal of Fluorescent Dye (CentriPure 50-Z25M)

  • 5.0 mL sample volume

  • Separation of fluorescein (490 nm) from ovalbumin (280 nm)

  • Elution with pure water

Desalting of Protein Solution (CentriPure 10-Z25M)

  • 1.0 mL sample volume

  • Removal of 0.8 M NaCl from anti-rabbit IgG (280 nm)

  • Elution with pure water

  • Blue line shows conductivity drop

Explore the Catalogs and Flyers

Zetadex Gel Filtration for Rapid Purification, Desalting, and Buffer Exchange
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