Select Page

Gravity Flow Gel Filtration for Rapid Desalting and Buffer Exchange

Dextran-Based Size Exclusion Columns and Plates for Proteins, Oligonucleotides, and Nanoparticles

CentriPure™ is emp BIOTECH’s versatile product line for desalting, buffer exchange, and purification using Zetadex™, a dextran-based size exclusion resin. Whether working with antibodies, oligonucleotides, enzymes, or nanoparticles, CentriPure™ enables the rapid and efficient removal of small molecules—such as salts, dyes, biotin, or haptens—from larger biomolecules in a single step.

The product range covers volumes from 150 µL to 100 ml and supports both manual and automated workflows. Whether you’re purifying antibodies, enzymes, oligonucleotides, or nanoparticles, CentriPure™ delivers high recovery with minimal dilution.

Contact Us

Key Features and Benefits of CentriPure™ Gel Filtration Columns

CentriPure™ columns deliver fast, reproducible desalting and buffer exchange across a broad range of sample volumes and applications. All columns are pre-packed with ultrapure Zetadex™ dextran-based resin and are ready to use—no resin preparation, equilibration, or setup required.

Pre-packed and ready to use
No setup, swelling, or resin handling—just equilibrate and purify.

Gravity-flow operation
Standalone workflow with no need for centrifuges or pumps.

Two MWCO options
Zetadex-25 (5 kDa / 10 bp) and Zetadex-50 (25 kDa / 20 bp) for targeted separation.

High recovery and resolution
≥85% recovery with efficient removal of salts, dyes, biotin, and haptens.

Scalable fixed-volume formats
Available in 11 sizes from 150 µL to 100 ml.

Endotoxin-free options
Certified low-endotoxin formats for sensitive workflows.

Explore the CentriPure™ Product Formats

CentriPure™ Columns

Gravity Flow Desalting from 150 µL to 100 ml

Pre-packed, fixed-volume columns for desalting and buffer exchange without centrifugation.

  • 11 sizes available

  • Elution volume = 1.5× sample volume

  • For proteins, nucleic acids, and nanoparticles

Learn more about the CentriPure gravity columns.

CentriPure™ MINI Columns

Spin Desalting up to ≤100 µL

Sterile, conical centrifugation columns for ultrafast cleanup of small-volume samples.

  • Removes up to 99.999% salts, dyes, biotin, and haptens

  • Processing time: under 5 minutes

Learn more about the CentriPure MIDI columns.

CentriPure™ MIDI Columns

Centrifugation Desalting — 500 to 700 µL

Hydrated columns with integrated adapter for secure and consistent spinning.

  • Removes up to 99.99% of small molecules

  • Ideal for dye-labeled proteins and oligos

Learn more about the CentriPure MIDI columns.

CentriPure™ Column Arrays

96-Well High-Throughput Desalting — 300 to 500 µL

SLAS-compatible arrays for automated parallel sample cleanup.

  • For robots, liquid handlers, or vacuum manifolds

  • Process 96 samples in parallel

Learn more about the CentriPure 96-well column arrays

CentriPure™ Plates

Desalting Plates — Up to 70 µL in 96- and 384-Well Formats

Designed for high-throughput labs using small sample volumes.

  • No dilution of sample

  • Optional drip directors prevent cross-contamination

Learn more about the CentriPure desalting plates for high-throughput labs.

ZetaSep™ Desalting Columns

Post-FPLC Buffer Exchange — 200 µL to 1 mL

Pre-packed columns for inline monitoring and polishing steps.

  • Pressure compatible up to 3 bar

  • Suitable for UV-based elution tracking

Learn more about the ZetaSep desalting.

Zetadex™ Resin: Consistent, High-Performance Separation

All CentriPure™ formats are powered by Zetadex™, a proprietary, cross-linked dextran gel filtration resin.

  • Zetadex-25: MWCO ~5 kDa (proteins) / 10 bases (nucleic acids)

  • Zetadex-50: MWCO ~25 kDa (proteins) / 20 bases (nucleic acids)

Ultrapure gel and specially treated frits ensure outstanding resolution, low sample loss, and minimal cross-contamination.

Reliable Dextran-Based Alternative to Sephadex® for Gel Filtration Applications

The emp BIOTECH offering provides a like-for-like alternative to established dextran-based media such as Sephadex® and other comparable gel filtration resins—delivering equivalent performance in size-based separation, chemical stability, and application versatility.

Protein and Antibody Purification

Removal of Fluorescent Dye (CentriPure 50-Z25M)

  • 5.0 mL sample volume

  • Separation of fluorescein (490 nm) from ovalbumin (280 nm)

  • Elution with pure water

Desalting of Protein Solution (CentriPure 10-Z25M)

  • 1.0 mL sample volume

  • Removal of 0.8 M NaCl from anti-rabbit IgG (280 nm)

  • Elution with pure water

  • Blue line shows conductivity drop

Explore the Catalogs and Flyers

Zetadex Gel Filtration for Rapid Purification, Desalting, and Buffer Exchange
[Download Now]